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KMID : 0616120160470010062
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 1 p.62 ~ p.72
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Quantitative Determination of the Thirteen Marker Components in Banhasasim-Tang Decoction Using an Ultra-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry
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Seo Chang-Seob
Shin Hyeun-Kyoo
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Abstract
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Banhasasim-tang is a well-known traditional Korean herbal formula and has been used clinically for the treatment of gastric disease, including acute and chronic gastritis, diarrhea and gastric ulcers in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer method was developed for the quantitative determination of the 13 marker constituents, homogentisic acid (1), 3,4-dihydroxybenzaldehyde (2), spinosin (3), liquiritin (4), baicalin (5), ginsenoside Rg1 (6), liquiritigenin (7), wogonoside (8), ginsenoside Rb1 (9), baicalein (10), glycyrrhizin (11), wogonin (12), and 6-gingerol (13) in Banhasasim-tang decoction. Separation of the compounds 1-13 was using an UPLC BEH C18 (100×2.1 mm, 1.7 mm) column and column oven temperature was maintained at 45oC. The mobile phase consisted of 0.1% (v/v) formic acid in water (A) and acetonitrile (B) by gradient elution. The injection volume and flow rate were 2.0 mL and 0.3 mL/min, respectively. Calibration curves of the compounds 1-13 were showed with r2 values ³ 0.9908. The limit of detection and limit of quantification values of the compounds 1-13 were 0.04-1.11 ng/mL and 0.13-3.33 ng/mL, respectively. Among the these compounds, the compounds 1-3 were not detected, while the compounds 4-13 were detected in the ranges of 3.20-107,062.98 mg/g in Banhasasim-tang sample.
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KEYWORD
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LC-MS/MS , Banhasasim-tang , Quantitative Determination
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